製品の概要

  • 製品名Anti-HOXA13 antibody
    HOXA13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HOXA13
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HOXA13.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HEK293 whole cell lysate and human skin FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 関連疾患Defects in HOXA13 are the cause of hand-foot-genital syndrome (HFGS) [MIM:140000]; also known as hand-foot-uterus syndrome. The clinical features include small feet with unusually short great toes and abnormal thumbs. Females with the disorder have duplication of the genital tract.
    Defects in HOXA13 are the cause of Guttmacher syndrome (GUTTS) [MIM:176305]. Guttmacher syndrome is a dominantly inherited combination of distal limb and genital tract abnormalities. It has several features in common with hand-foot-genital syndrome, including hypoplastic first digits and hypospadias. Typical features not seen in hand-foot-genital syndrome include postaxial polydactyly of the hands and uniphalangeal second toes with absent nails.
  • 配列類似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態This protein has also been described in cytoplasm.
  • 別名
    • Homeo box 1J antibody
    • Homeo box A13 antibody
    • Homeobox 1J antibody
    • Homeobox A13 antibody
    • Homeobox protein Hox A13 antibody
    • Homeobox protein Hox-1J antibody
    • Homeobox protein Hox-A13 antibody
    • Homeobox protein HOXA13 antibody
    • Homeobox1J antibody
    • HomeoboxA13 antibody
    • HOX 1 antibody
    • HOX 1J antibody
    • Hox 1J protein antibody
    • HOX A13 antibody
    • HOX1 antibody
    • HOX1J antibody
    • HOXA 13 antibody
    • HOXA13 antibody
    • HXA13_HUMAN antibody
    • Transcription factor HOXA13 antibody
    see all

Anti-HOXA13 antibody 画像

  • All lanes : Anti-HOXA13 antibody (ab106503) at 1 µg/ml

    Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
    Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of ab106503 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106503, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HOXA13 antibody (ab106503) 使用論文

This product has been referenced in:
  • Cantile M  et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . Read more (PubMed: 24189220) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"