製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab28699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.

ターゲット情報

  • 機能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 関連疾患Radioulnar synostosis with amegakaryocytic thrombocytopenia
  • 配列類似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homeo box 1I antibody
    • Homeo box A11 antibody
    • Homeobox A11 antibody
    • Homeobox protein Hox A11 antibody
    • Homeobox protein Hox-1I antibody
    • Homeobox protein Hox-A11 antibody
    • Homeobox protein HOXA11 antibody
    • Hox 1I antibody
    • HOX1 antibody
    • HOX1I antibody
    • HOXA11 antibody
    • HXA11_HUMAN antibody
    see all

Anti-HOXA11 antibody 画像

  • ICC/IF image of ab28699 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab28699, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

Anti-HOXA11 antibody (ab28699) 使用論文

This product has been referenced in:
  • Connell KA  et al. HOXA11 promotes fibroblast proliferation and regulates p53 in uterosacral ligaments. Reprod Sci 16:694-700 (2009). Read more (PubMed: 19372592) »
  • Connell KA  et al. HOXA11 is critical for development and maintenance of uterosacral ligaments and deficient in pelvic prolapse. J Clin Invest 118:1050-5 (2008). IHC-P ; Human . Read more (PubMed: 18274672) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"