Anti-Hornerin 抗体 (ab78909)
Key features and details
- Goat polyclonal to Hornerin
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Hornerin antibody -
製品の詳細
Goat polyclonal to Hornerin -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Hornerin aa 17-30 (internal sequence).
Sequence:C-QYATQHGEYDTLNK
Database link: NP_001009931.1 -
ポジティブ・コントロール
- ICC/IF: U251 cells. Flow Cyt (intra): A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab78909 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78909の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
|
特記事項 |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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関連性
Hornerin has similar structural features to those of profilaggrin, an essential protein for keratinization of epidermal tissues. Hornerin may play a role in cornification. -
細胞内局在
Cytoplasmic granule. Note: Found in keratohyalin granules in the granular cells of the epidermis -
参照データベース
- Entrez Gene: 388697 Human
- SwissProt: Q86YZ3 Human
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別名
- 1110033K19Rik antibody
- AV011854 antibody
- Intermediate filament protein antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab78909) for 1hr at 10µg/ml followed by Alexa Fluor® 488 secondary antibody at 2µg/ml. shows cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor® 488 secondary antibody (2µg/ml).
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Flow cytometric analysis of A549 cells labeling Hornerin with ab78909 at 10ug/mL (blue line). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Followed by Alexa Fluor 488 secondary antibody (1ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab78909 は 2 報の論文で使用されています。
- Bilandzic M et al. Keratin-14 (KRT14) Positive Leader Cells Mediate Mesothelial Clearance and Invasion by Ovarian Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 31443478
- Gutknecht MF et al. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity. Nat Commun 8:552 (2017). PubMed: 28916756