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RabMAb

Anti-Hormone sensitive lipase (phospho S855) 抗体 [EPR2330] (ab76492)

製品の概要

  • 製品名
    Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330]
    Hormone sensitive lipase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2330] to Hormone sensitive lipase (phospho S855)
  • 特異性
    Detects HSL/LIPE only when phosphorylated on Serine 855
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Phospho-specific peptide corresponding to residues surrounding Serine 855 of Human HSL/LIPE

  • ポジティブ・コントロール
    • Human skeletal muscle lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

アプリケーション

Our Abpromise guarantee covers the use of ab76492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/1000 - 1/5000. Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2).

    Is unsuitable for ICC/IF, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
    • パスウェイ
      Glycerolipid metabolism; triacylglycerol degradation.
    • 配列類似性
      Belongs to the 'GDXG' lipolytic enzyme family.
    • 細胞内局在
      Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Hormone sensitive lipase antibody
      • Hormone sensitive lipase testicular isoform antibody
      • Hormone-sensitive lipase antibody
      • HSL antibody
      • LHS antibody
      • Lipase hormone sensitive antibody
      • LIPE antibody
      • LIPS_HUMAN antibody
      see all

    画像

    • All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution

      Lane 1 : Human skeletal muscle lysate, untreated
      Lane 2 : Human skeletal muscle lysate, treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 117 , 83 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab76492 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for your inquiry.

    The Serine 855 of human HSL is equivalent to Serine 559 of mouse HSL, and this would recognize the Serine 559 of mouse HSL. The orthologous residue for isoform 2 of the rat HSL would be Serine 565, and this antibo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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