Anti-Hormone sensitive lipase (phospho S853) 抗体 [EPR2329(2)] (ab109400)
製品の概要
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製品名Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)]
Hormone sensitive lipase 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase (phospho S853)
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由来種Rabbit
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特異性The antibody only detects HSL phosphorylated on serine 853.
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アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP -
種交差性交差種: Mouse, Rat, Human
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免疫原
Phospho-specific peptide corresponding to residues surrounding serine 853 of Human HSL/LIPE (UniProt Q05469).
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ポジティブ･コントロール
- Human skeletal muscle lysates and Mouse muscle lysates
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR2329(2)
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アイソタイプIgG
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研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
アプリケーション
Our Abpromise guarantee covers the use of ab109400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa). |
ターゲット情報
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機能In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
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パスウェイGlycerolipid metabolism; triacylglycerol degradation.
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配列類似性Belongs to the 'GDXG' lipolytic enzyme family.
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細胞内局在Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
- Information by UniProt
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参照データベース
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Entrez Gene: 25330 Rat
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- SwissProt: P15304 Rat
see all -
別名
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
画像
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![Western blot - Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400)](http://a.abcam.com/ps/products/109/ab109400/Images/HSLLIPE-Primary-antibodies-ab109400-1.jpg)
Western blot - Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400)All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa (why is the actual band size different from the predicted?) -
![Western blot - Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400)](http://a.abcam.com/ps/products/109/ab109400/Images/HSLLIPE-Primary-antibodies-ab109400-2.jpg)
Western blot - Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400)All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa (why is the actual band size different from the predicted?)
プロトコール
参考文献
ab109400 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"