The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Involved in meiotic progression.
組織特異性Expressed in testis, liver and brain, and at lower levels in kidney.
配列類似性Contains 1 HORMA domain.
細胞内局在Nucleus. Chromosome. Preferentially localizes to unsynapsed or desynapsed chromosomal regions during the male and female prophase I stage of meiosis. TRIP13 is required for depletion from synapsed chromosomes.