製品の概要

  • 製品名Anti-HOOK1 antibody
    HOOK1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to HOOK1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: MLLRKQLAEK ERRIEILESE CKVAKFRDYE EKLIVSAWYN KSLAFQKLGM ESRLVSGGGA CSDTGACTPA RSFLAQQRHI TNTRRNLSVK VPATTSD, corresponding to amino acids 632-728 of Human HOOK1 (NP_056972) with tag.

  • ポジティブ・コントロール
    • MCF-7 cell lysate; Recombinant tagged human HOOK1 fragment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
  • 配列類似性Belongs to the hook family.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A930033L17Rik antibody
    • Abnormal spermatozoon head shape antibody
    • azh antibody
    • h-hook1 antibody
    • hHK1 antibody
    • HK1 antibody
    • HOOK 1 antibody
    • Hook homolog 1 (Drosophila) antibody
    • Hook1 antibody
    • HOOK1_HUMAN antibody
    • MGC10642 antibody
    • OTTHUMP00000010548 antibody
    • OTTMUSP00000008480 antibody
    • Protein Hook homolog 1 antibody
    • RP23-80B16.4 antibody
    see all

Anti-HOOK1 antibody 画像

  • Anti-HOOK1 antibody (ab77422) at 1 µg/ml + Recombinant tagged human HOOK1 fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 85 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-HOOK1 antibody (ab77422) at 1 µg/ml + MCF-7 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 85 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-HOOK1 antibody (ab77422) 使用論文

ab77422 has not yet been referenced specifically in any publications.

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