Using conjugated Homocysteine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:Compound : Cross-reactivity ratio(a)
Homocysteic acid-G-BSA: 1/>100000
(a) Homocysteine-G-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement
G = Glutaraldehyde, BSA = Bovine Serum Albumin
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For ELISA, dilute 1:2000 - 1:10000 in PBS.
Optimal dilutions should be determined by the individual
Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group.
Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine.
(2S) 2 amino 4 sulfanyl butanoic acid antibody
This product has been referenced in:
Ganapathy PS et al. Endogenous elevation of homocysteine induces retinal neuron death in the cystathionine-beta-synthase mutant mouse. Invest Ophthalmol Vis Sci50:4460-70 (2009).
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