A region within a synthetic peptide: WFGNKRIRYK KNIGKAQEEA NLYAAKKAAG ASPYSMAGPP SGTTTPMMSP, corresponding to C terminal amino acids 288-327 of Fruit fly (Drosophila melanogaster) Homeobox protein extradenticle
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Homeobox protein extradenticle belongs to the TALE/PBX homeobox family and contains 1 homeobox DNA-binding domain. It is a transcription factor which acts with the selector homeodomain proteins altering the regulation of downstream target genes such as wingless, teashirt and decapentaplegic. It is expressed both maternally and zygotically at all stages of development and affects segmental identity.