Anti-Holocytochrome C 抗体 (ab2542)

製品の概要

  • 製品名Anti-Holocytochrome C antibody
  • 製品の詳細
    Mouse monoclonal to Holocytochrome C
  • 特異性This antibody detects a 12 kDa protein corresponding to the apparent molecular weight of cytochrome c. The antibody is specific for holocytochrome c. It does not detect apocytochrome c.
  • アプリケーション適用あり: ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    非交差種: Horse, Guinea pig, Cow, Pig
  • 免疫原

    Cytochrome c purified from human heart.

  • 特記事項


    The precursor of cytochrome c, apocytochrome c, is synthesized in the cytoplasm. Upon translocation into mitochondria, cytochrome c acquires a heme moiety required for functionality in the mitochondrial respiration chain. The heme-bound form of cytochrome c is called holocytochrome c. When released into the cytosol, cytochrome c can activate the caspases responsible for apoptosis.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 6 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 - 10 µg/ml.

Anti-Holocytochrome C antibody 画像

  • ab2542 staining differentiating (rat) PC12 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 6 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.  Cells were counterstained with the nuclear Topro-3 stain.

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Anti-Holocytochrome C antibody (ab2542) 使用論文

ab2542 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (differentiating PC12 cells)
Specification differentiating PC12 cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X100 for 5 minutes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Anne Chiaramello

Verified customer

投稿 Apr 18 2008

Application Western blot
Sample Rice Cell lysate - whole cell (THP1 cells)
Specification THP1 cells
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Nov 14 2005

Yes, the antibody is specific for holocytochrome c. It does not detect apocytochrome c. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"