製品の概要

  • 製品名Anti-HNRPUL1 antibody [EP2633Y]
    HNRPUL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2633Y] to HNRPUL1
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C-terminus of human HNRPUL1.

  • ポジティブ・コントロール
    • HeLa cell lysate, HeLa cells and human brain tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
IP 1/40.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/80. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
  • 配列類似性Contains 1 B30.2/SPRY domain.
    Contains 1 SAP domain.
  • ドメインThe RGG-box domain is methylated.
  • 翻訳後修飾Methylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenovirus early region 1B associated protein 5 antibody
    • Adenovirus early region 1B-associated protein 5 antibody
    • E130317O14Rik antibody
    • E1B 55 kDa associated protein 5 antibody
    • E1B AP5 antibody
    • E1B-55 kDa-associated protein 5 antibody
    • E1B-AP5 antibody
    • E1BAP5 antibody
    • fb53d10 antibody
    • fk45c03 antibody
    • FLJ12944 antibody
    • Heterogeneous nuclear ribonucleoprotein U-like protein 1 antibody
    • HNRL1_HUMAN antibody
    • Hnrnpul1 antibody
    • HNRPUL1 antibody
    • MGC36621 antibody
    • MGC78330 antibody
    • wu:fb53d10 antibody
    • wu:fk45c03 antibody
    • zgc:85971 antibody
    see all

Anti-HNRPUL1 antibody [EP2633Y] 画像

  • Anti-HNRPUL1 antibody [EP2633Y] (ab68480) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit, HRP conjugated at 1/2000 dilution

    Predicted band size : 96 kDa
    Observed band size : 96 kDa
    Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.
  • ab68480 at 1/100 dilution staining HNRPUL1 in HeLa cells by Immunocytochemistry.
  • ab68480 at 1/250 dilution staining HNRPUL1 in human brain by Immunohistochemistry, Paraffin-embedded tissue.
  • Overlay histogram showing HeLa cells stained with ab68480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68480, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-HNRPUL1 antibody [EP2633Y] (ab68480) 使用論文

This product has been referenced in:
  • Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . Read more (PubMed: 25299183) »
  • Massimelli MJ  et al. Interplay between polyadenylate-binding protein 1 and Kaposi's sarcoma-associated herpesvirus ORF57 in accumulation of polyadenylated nuclear RNA, a viral long noncoding RNA. J Virol 87:243-56 (2013). Read more (PubMed: 23077296) »

See all 2 Publications for this product

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