製品の概要

  • 製品名Anti-HNRPH3 antibody
    HNRPH3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HNRPH3
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: GLEIVPNGIT LTMDYQGRST GEAFVQFASK EIAENALGKH KERIGHRYIE , corresponding to amino acids 38-87 of Human HNRPH3

  • ポジティブ・コントロール
    • Raji cell lysate. Human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 関連性HNRPH3 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • 2H9 antibody
    • FLJ34092 antibody
    • Heterogeneous nuclear ribonucleoprotein H3 antibody
    • hnRNP 2H9 antibody
    • hnRNP H3 antibody
    • HNRNPH 3 antibody
    • HNRNPH3 antibody
    • HNRPH 3 antibody
    see all

Anti-HNRPH3 antibody 画像

  • All lanes : Anti-HNRPH3 antibody (ab66663) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Raji cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 17 kDa,35 kDa (possible isoform). We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human kidney tissue labelling HNRPH3 with ab66663 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnifciation: 400X.

Anti-HNRPH3 antibody (ab66663) 使用論文

ab66663 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control hnRNP C (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A Dynabeads
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投稿 Jun 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"