製品の概要
- 製品名Anti-HNRPA3 antibody
HNRPA3 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to HNRPA3
- アプリケーション適用あり: WB, IHC-Pmore details
- 種交差性交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Dog
- 免疫原
A region within synthetic peptide: MEVKPPPGRP QPDSGRRRRR RGEEGHDPKE PEQLRKLFIG GLSFETTDDS, corresponding to N terminal amino acids 1-50 of Human HNRPA3
- ポジティブ･コントロール
- IHC-P: Human liver and human lung tissues WB: Jurkat cell lysate.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - 精製度Protein A purified
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab50949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
ターゲット情報
- 関連性HNRPA3 plays a role in cytoplasmic trafficking of RNA. It binds to the cis-acting response element(A2RE) and may be involved in pre-mRNA splicing.
- 細胞内局在Nuclear
-
参照データベース
- Entrez Gene: 220988 Human
- Entrez Gene: 229279 Mouse
- Entrez Gene: 362152 Rat
- Omim: 605372 Human
- SwissProt: P09867 Cow
- SwissProt: P51991 Human
- SwissProt: Q8BG05 Mouse
- SwissProt: Q6URK4 Rat
see all -
別名
- FBRNP antibody
- Heterogeneous nuclear ribonucleoprotein A3 antibody
- HNRPA3 antibody
Anti-HNRPA3 antibody 画像
-
Western blot - Anti-HNRPA3 antibody (ab50949)All lanes : Anti-HNRPA3 antibody (ab50949) at 0.625 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with blocking peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Predicted band size : 40 kDa -
Immunohistochemistry (Paraffin-embedded sections) - HNRPA3 antibody (ab50949)Tissue: Paraffin embedded human liver
Cells with positive label: Hepatocytes (indicated with arrows)
Antibody concentration: 4µg/ml
Magnification: 400XTissue: Paraffin embedded human liver
Cells with positive label: Hepatocytes (indicated with arrows)
Antibody concentration: 4µg/ml
Magnification: 400X -
Western blot - HNRPA3 antibody (ab50949)Anti-HNRPA3 antibody (ab50949) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG at 1/50,000
Predicted band size : 40 kDa
Observed band size : 40 kDa
Buffer: 5% skim milk / PBS
Gel concentration: 12% -
Immunohistochemistry (Paraffin-embedded sections) - HNRPA3 antibody (ab50949)Tissue: Paraffin-embedded human lung
Cells with positive label: Alveolar cells (indicated with arrows)
Antibody concentration: 4 µg/ml
Magnification: 400XTissue: Paraffin-embedded human lung
Cells with positive label: Alveolar cells (indicated with arrows)
Antibody concentration: 4 µg/ml
Magnification: 400X
Anti-HNRPA3 antibody (ab50949) 使用論文
This product has been referenced in:
- Roda D et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One 10:e0130543 (2015). Human . Read more (PubMed: 26110767) »
- Papadopoulou C et al. Extensive association of HuR with hnRNP proteins within immunoselected hnRNP and mRNP complexes. Biochim Biophys Acta 1804:692-703 (2010). Read more (PubMed: 19931428) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"