製品の概要

  • 製品名
    Anti-HNRPA3 antibody
    HNRPA3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HNRPA3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog
  • 免疫原

    A region within synthetic peptide: MEVKPPPGRP QPDSGRRRRR RGEEGHDPKE PEQLRKLFIG GLSFETTDDS, corresponding to N terminal amino acids 1-50 of Human HNRPA3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • IHC-P: Human liver and human lung tissues WB: Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

画像

  • Western blot - Anti-HNRPA3 antibody (ab50949)
    Western blot - Anti-HNRPA3 antibody (ab50949)
    All lanes : Anti-HNRPA3 antibody (ab50949) at 0.625 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 40 kDa

  • Immunohistochemistry (Paraffin-embedded sections) - HNRPA3 antibody (ab50949)
    Immunohistochemistry (Paraffin-embedded sections) - HNRPA3 antibody (ab50949)
    Tissue: Paraffin embedded human liver
    Cells with positive label: Hepatocytes (indicated with arrows)
    Antibody concentration: 4µg/ml
    Magnification: 400X

    Tissue: Paraffin embedded human liver
    Cells with positive label: Hepatocytes (indicated with arrows)
    Antibody concentration: 4µg/ml
    Magnification: 400X
  • Western blot - HNRPA3 antibody (ab50949)
    Western blot - HNRPA3 antibody (ab50949)
    Anti-HNRPA3 antibody (ab50949) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG at 1/50,000

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Buffer: 5% skim milk / PBS
    Gel concentration: 12%
  • Immunohistochemistry (Paraffin-embedded sections) - HNRPA3 antibody (ab50949)
    Immunohistochemistry (Paraffin-embedded sections) - HNRPA3 antibody (ab50949)
    Tissue: Paraffin-embedded human lung
    Cells with positive label: Alveolar cells (indicated with arrows)
    Antibody concentration: 4 µg/ml
    Magnification: 400X

    Tissue: Paraffin-embedded human lung
    Cells with positive label: Alveolar cells (indicated with arrows)
    Antibody concentration: 4 µg/ml
    Magnification: 400X

参考文献

This product has been referenced in:
  • Roda D  et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One 10:e0130543 (2015). Human . Read more (PubMed: 26110767) »
  • Papadopoulou C  et al. Extensive association of HuR with hnRNP proteins within immunoselected hnRNP and mRNP complexes. Biochim Biophys Acta 1804:692-703 (2010). Read more (PubMed: 19931428) »

See all 3 Publications for this product

ab50949 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Western blot abreview for Anti-HNRPA3 antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount
50 µg
Specification
Mouse embryonic fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
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投稿 Jan 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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