Anti-hnRNP U/p120 抗体 (ab122906)
Key features and details
- Rabbit polyclonal to hnRNP U/p120
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-hnRNP U/p120 antibody
hnRNP U/p120 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP U/p120 -
由来種
Rabbit -
特異性
ab122906 can recognize exogenous Myc tagged hnRNP U/p120 (vector expressed hnRNP U/p120) and endogenous hnRNP U/p120 (native). -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122906の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/3000. Predicted molecular weight: 91 kDa.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/1000 - 1/3000. Predicted molecular weight: 91 kDa. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. -
配列類似性
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
翻訳後修飾
Extensively phosphorylated.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 3192 Human
- Entrez Gene: 51810 Mouse
- Omim: 602869 Human
- SwissProt: Q00839 Human
- SwissProt: Q8VEK3 Mouse
- Unigene: 106212 Human
- Unigene: 86589 Mouse
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別名
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
see all
画像
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Lanes 1-2 : anti Myc antibody
Lanes 3-4 : Anti-hnRNP U/p120 antibody (ab122906) at 1/1000 dilution
Lanes 1 & 3 : lysates from SAOS2 cells transfected with pRc/CMV vector
Lanes 2 & 4 : lysates from SAOS2 cells transfected with pRc/CMV vector expressing Myc tagged hnRNP U
Predicted band size: 91 kDaMyc - hnRNP U/p120: Exogenous Myc tagged hnRNP U/p120
hnRNP U/p120: endogenous hnRNP U/p120. -
Immunofuorescent analysis of hnRNP U/p120 expression using ab122906 in Mouse P19 neurons (left) and in cells with in situ extracted nuclear matrix (right). Note that hnRNP U is concentrated in intranuclear speckles throughout the nucleoplasm.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab122906 は 1 報の論文で使用されています。
- Taniura H & Yoshikawa K Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix. J Cell Biochem 84:545-55 (2002). PubMed: 11813259