Anti-hnRNP K (phospho S284) 抗体 (ab74066)
Key features and details
- Rabbit polyclonal to hnRNP K (phospho S284)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-hnRNP K (phospho S284) antibody
hnRNP K 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP K (phospho S284) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human hnRNP K (phospho S284).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74066の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
配列類似性
Contains 3 KH domains. -
翻訳後修飾
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 15387 Mouse
- SwissProt: P61979 Mouse
- Unigene: 142872 Mouse
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別名
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
画像
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All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution
Lane 1 : Extracts from 3T3 cells treated
with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from 3T3 cells treated
with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab74066 は 1 報の論文で使用されています。
- Zhao L et al. The anticancer effects of cinobufagin on hepatocellular carcinoma Huh-7 cells are associated with activation of the p73 signaling pathway. Mol Med Rep 19:4119-4128 (2019). PubMed: 30942456