製品の概要

  • 製品名Anti-hnRNP K antibody [EP943Y]
    hnRNP K 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP943Y] to hnRNP K
  • 特異性This antibody detects both phosphorylated and unphosphorylated hnRNP K.
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human hnRNP.

  • ポジティブ・コントロール
    • Jurkat cell lysate; HeLa cells; Human breast carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-hnRNP K antibody (Alexa Fluor® 488) [EP943Y] (ab203598)

    Anti-hnRNP K antibody (Alexa Fluor® 647) [EP943Y] (ab203599)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP943Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • 配列類似性Contains 3 KH domains.
  • 翻訳後修飾Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Anti-hnRNP K antibody [EP943Y] 画像

  • Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 51 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
  • Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
  • ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.

    For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-hnRNP K antibody [EP943Y] (ab52600) 使用論文

This product has been referenced in:
  • Rogée S  et al. Quantitative proteomics identifies host factors modulated during acute hepatitis E virus infection in the swine model. J Virol 89:129-43 (2015). WB ; Pig . Read more (PubMed: 25320303) »
  • Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . Read more (PubMed: 25299183) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal colon)
Specification Normal colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"