製品の概要

  • 製品名Anti-hnRNP H antibody
    hnRNP H 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to hnRNP H
  • 特異性The antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
  • アプリケーション適用あり: WB, IP, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human heterogeneous nuclear ribonucleoprotein H1 (LocusLink ID 3187).

  • ポジティブ・コントロール
    • nuclear lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibodies were affinity purified using the peptide immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
IP Use at 4-8 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 24462217
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500. PubMed: 20020773

ターゲット情報

  • 機能This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
  • 組織特異性Expressed ubiquitously.
  • 配列類似性Contains 3 RRM (RNA recognition motif) domains.
  • ドメインEach quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
  • 細胞内局在Nucleus > nucleoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686A15170 antibody
    • Heterogeneous nuclear ribonucleoprotein H antibody
    • Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • HNRH1_HUMAN antibody
    • hnRNP H antibody
    • hnRNPH antibody
    • Hnrnph1 antibody
    • HNRPH 1 antibody
    • HNRPH antibody
    • HNRPH1 protein antibody
    • N-terminally processed antibody
    see all

Anti-hnRNP H antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 53 kDa

    ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40 µg) from HeLa cells and recombinant human hnRNPH (rH; ~100 ng). Antibody: ab10374 used at the indicated concentrations. Detection: Protein A – HRP with chemiluminescence (20 second exposure after a 20 minute delay to allow signal to decay).

    ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40 µg) from HeLa cells and recombinant human hnRNPH (rH; ~100 ng). Antibody: ab10374 used at the indicated concentrations. Detection: Protein A – HRP with chemiluminescence (20 second exposure after a 20 minute delay to allow signal to decay).
  • ICC/IF image of ab10374 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10374, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab10374 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10374, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-hnRNP H antibody (ab10374) 使用論文

This product has been referenced in:
  • Juan WC  et al. Identification of cis-Acting Elements and Splicing Factors Involved in the Regulation of BIM Pre-mRNA Splicing. PLoS One 9:e95210 (2014). WB . Read more (PubMed: 24743263) »
  • Cortese A  et al. Widespread RNA metabolism impairment in sporadic inclusion body myositis TDP43-proteinopathy. Neurobiol Aging 35:1491-8 (2014). IHC-Fr ; Human . Read more (PubMed: 24462217) »

See all 11 Publications for this product

Product Wall

Thank you for contacting Abcam. We have not tested this antibody specifically with hnRNP F/A/B. However, It is unlikely that this antibody will react with the other family members. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (HeLa cytosolic extract)
Total protein in input 500 µg
Specification HeLa cytosolic extract
Immuno-precipitation step Protein A
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Abcam user community

Verified customer

投稿 Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount 50 µg
Specification HeLa cytosolic and nuclear extracts
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Oct 30 2009

Thank you for your enquiry. The epitope recognized by ab10374 has been mapped to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneI...

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Thank you for your enquiry. The antibody ab10374 was designed to recognize hnRNPH1 and does not recognize hnRNPH3. This information has now been added on our datasheet. Please do not hesitate to contact us again if you require further information,

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I'm sorry to hear you are experiencing a problem with ab10374 in WB. You are absolutely correct, there was indeed an error on the datasheet and it has been corrected to " use at a dilution of 1:10 000 to 1:30 000, therefore this would be equivalent...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"