製品の概要

  • 製品名Anti-hnRNP F+H antibody [1G11]
  • 製品の詳細
    Mouse monoclonal [1G11] to hnRNP F+H
  • 特異性Human hnRNP F/H.
  • アプリケーション適用あり: IHC-P, ELISA, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human, Xenopus laevis
  • 免疫原

    Unfortunately, this information is considered to be commercially sensitive

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 53 kDa (hnRNP F), 56 kDa (hnRNP H). (predicted molecular weight: hnRNP F = 49.8 kDa, hnRNP H = 53.9kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性The hnRNPs belong to a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties.
  • 参照データベース
  • 別名
    • Heterogeneous nuclear ribonucleoprotein F antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • hnRNP F antibody
    • hnRNP H antibody
    • HNRNPF antibody
    • HNRNPH1 antibody
    • HNRPH1 antibody
    • mcs94 1 antibody
    • Nucleolin like protein antibody
    • Nucleolin like protein mcs94 1 antibody
    see all

Anti-hnRNP F+H antibody [1G11] 画像

  • IHC image of ab10689 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10689, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-hnRNP F+H antibody [1G11] (ab10689) 使用論文

This product has been referenced in:
  • Cooper-Knock J  et al. Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions. Brain 137:2040-51 (2014). IHC-P ; Human . Read more (PubMed: 24866055) »
  • Mandler MD  et al. A cytoplasmic quaking I isoform regulates the hnRNP F/H-dependent alternative splicing pathway in myelinating glia. Nucleic Acids Res 42:7319-29 (2014). WB ; Mouse . Read more (PubMed: 24792162) »

See all 10 Publications for this product

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Thank you very much for your review of ab10689.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - nuclear (Brain)
Loading amount 40 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 20 2012

DISCOUNT CODE: ***
Expiration date: November 7, 2012

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Sinceab10689 has not been testedwith mouse samples,I can offer a discount off a future purchase if you buy ab10689 now, test itwith mouse samplesand submit feedback to us in the form of a...

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Thank you for contacting Abcam regarding the IF protocol for ab10689. If you are staining cells, I have provided two protocols below, depending on your fixation method.  If you are staining tissue, the protocol is available on the datasheet (under ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (HeLa cytosolic extract)
Total protein in input 500 µg
Specification HeLa cytosolic extract
Immuno-precipitation step Protein G
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投稿 Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount 50 µg
Specification HeLa cytosolic and nuclear extracts
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"