The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).Can be blocked with hnRNP F peptide (ab197136). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Use a concentration of 4 - 8 µg/ml.
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Contains 3 RRM (RNA recognition motif) domains.
The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.
Phosphorylated upon DNA damage, probably by ATM or ATR. Sumoylated.
Immunohistochemistry (Paraffin-embedded sections) - hnRNP F antibody (ab50982)
ab50982, at a concentration of 4 µg/ml, staining hnRNP F in paraffin embedded human kidney tissue sections by Immunohistochemistry. Cells with positive label are epithelial cells of renal tubule (indicated by arrows). Magnification: 400x.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP F antibody (ab50982)
ICC/IF image of ab50982 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50982, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP F antibody (ab50982)Image from White R et al, J Biol Chem. 2012 Jan 13;287(3):1742-54. Epub 2011 Nov 29, Fig 1. DOI 10.1074/jbc.M111.235010 January 13, 2012 The Journal of Biological Chemistry, 287, 1742-1754.
ab50982 staining hnRNP F in primary mouse oligodendrocytes (image shows 4 days in vitro) by Immunocytochemistry/ Immunofluorescence. hnRNP F is present in the nucleus and the cytoplasm of oligodendrocytes. In addition to its prominent nuclear localization (yellow arrowheads), hnRNP F is present in granular structures throughout the cytoplasm (white arrows). Insets show enlarged areas.
Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One9:e108625 (2014).
Read more (PubMed: 25299183) »
White R et al. Heterogeneous Nuclear Ribonucleoprotein (hnRNP) F Is a Novel Component of Oligodendroglial RNA Transport Granules Contributing to Regulation of Myelin Basic Protein (MBP) Synthesis. J Biol Chem287:1742-54 (2012).
Read more (PubMed: 22128153) »