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Our Abpromise guarantee covers the use of ab6102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||1/100. PubMed: 17067748Fix with acetone.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/1500 - 1/6000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml.|
ab6102 staining hnRNP A2B1 in human A549 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocking with 10% serum at 200C for 1 hour was performed. Samples were incubated with primary antibody (1/100: in PBS containing 10% FCS) for 1 hour at 200C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.