Anti-HNF-4-alpha + HNF-4-gamma 抗体 (ab64911)

製品の概要

  • 製品名Anti-HNF-4-alpha + HNF-4-gamma antibody
  • 製品の詳細
    Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma
  • 特異性Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma antibody detects endogenous levels of total HNF-4-alpha/gamma protein.
  • アプリケーション適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human HNF-4-alpha + HNF-4-gamma (internal sequence).

  • ポジティブ・コントロール
    • Extracts from HeLa cells, HepG2 cells and 293 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Anti-HNF-4-alpha + HNF-4-gamma antibody 画像

  • All lanes : Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HepG2 cells
    Lane 3 : extracts from 293 cells
    Lane 4 : extracts from 293 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 30 kDa,40 kDa,65 kDa,75 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64911 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64911, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911) 使用論文

ab64911 has not yet been referenced specifically in any publications.

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