Anti-hnRNP R 抗体 (ab30930)
Key features and details
- Rabbit polyclonal to hnRNP R
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-hnRNP R antibody
hnRNP R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP R -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Zebrafish -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human hnRNP R.
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ポジティブ・コントロール
- WB: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate, NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate , MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate , Testis (Mouse) Tissue Lysate - normal tissue , Ovary (Mouse) Tissue Lysate - normal tissue , PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate. ICC/IF: HeLa
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (1) |
1/500. Detects a band of approximately 68, 73, 76 kDa (predicted molecular weight: 71 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/500. Detects a band of approximately 68, 73, 76 kDa (predicted molecular weight: 71 kDa). |
ターゲット情報
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機能
Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. -
配列類似性
Contains 3 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 10236 Human
- Entrez Gene: 74326 Mouse
- Entrez Gene: 319110 Rat
- Entrez Gene: 406735 Zebrafish
- Omim: 607201 Human
- SwissProt: O43390 Human
- Unigene: 373763 Human
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別名
- FLJ25714 antibody
- Heterogeneous nuclear ribonucleoprotein R antibody
- hnRNP R antibody
see all
画像
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ab30930 staining hnRNP R in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab30930 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-hnRNP R antibody (ab30930) at 1 µg/ml + HeLa Nuclear lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 71 kDaBlocking buffer: 2% BSA
Gel type: MOPS
Observed band size: 73, 77 and 80 kDa
Exposure Time: 1 minute
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All lanes : Anti-hnRNP R antibody (ab30930) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 73,77,80 kDa why is the actual band size different from the predicted?
The three bands observed are thought to be differentially processed forms of hnRNP R.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab30930 は 10 報の論文で使用されています。
- Pacetti M et al. Physiological tissue-specific and age-related reduction of mouse TDP-43 levels is regulated by epigenetic modifications. Dis Model Mech 15:N/A (2022). PubMed: 35243489
- Buratti E et al. Deferoxamine mesylate improves splicing and GAA activity of the common c.-32-13T>G allele in late-onset PD patient fibroblasts. Mol Ther Methods Clin Dev 20:227-236 (2021). PubMed: 33426149
- Ghanawi H et al. Loss of full-length hnRNP R isoform impairs DNA damage response in motoneurons by inhibiting Yb1 recruitment to chromatin. Nucleic Acids Res 49:12284-12305 (2021). PubMed: 34850154
- Gittings LM et al. Heterogeneous nuclear ribonucleoproteins R and Q accumulate in pathological inclusions in FTLD-FUS. Acta Neuropathol Commun 7:18 (2019). PubMed: 30755280
- Chen EB et al. HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis. Aging (Albany NY) 11:7473-7491 (2019). PubMed: 31527303
- Dombert B et al. Presynaptic localization of Smn and hnRNP R in axon terminals of embryonic and postnatal mouse motoneurons. PLoS One 9:e110846 (2014). PubMed: 25338097
- Glinka M et al. The heterogeneous nuclear ribonucleoprotein-R is necessary for axonal {beta}-actin mRNA translocation in spinal motor neurons. Hum Mol Genet : (2010). Zebrafish . PubMed: 20167579
- Kim C et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta 1800:977-85 (2010). WB ; Human . PubMed: 20541591
- Lim Y et al. Proteomic identification and comparative analysis of asymmetrically arginine-methylated proteins in immortalized, young and senescent cells. Electrophoresis 31:3823-33 (2010). WB ; Human . PubMed: 21080485
- Hadian K et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem 284:33384-91 (2009). WB ; Human . PubMed: 19808671