Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (human corneal endothelial cells in vitro)
Specification
human corneal endothelial cells in vitro
Fixative
Formaldehyde
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2% BSA 2% goat serum
Secondary antibody
Name
Non-Abcam antibody was used: alexa fluor 488 goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Zhiguo He
Verified customer
投稿 Nov 06 2012