Application
Western blot
Sample
Mouse Cell lysate - whole cell (c2c12 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
c2c12 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in PBST
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 53 kDa
Additional data
Additional Notes
Both lanes 1 and 2 are from the TA muscle of WT mice
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投稿 Nov 20 2017