Anti-hnRNP D/AUF1 抗体 (ab61193)
Key features and details
- Rabbit polyclonal to hnRNP D/AUF1
- Suitable for: ELISA, IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-hnRNP D/AUF1 antibody
hnRNP D/AUF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP D/AUF1 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IP, WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic non-phosphopeptide (Human) from around the phosphorylation site of serine 83 (NSSPPR)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61193の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration. PubMed: 20435889
|
|
WB |
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
|
|
ICC/IF |
1/500 - 1/1000.
|
|
IHC-P |
1/50 - 1/100.
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. PubMed: 20435889 |
WB
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
1/500 - 1/1000. |
IHC-P
1/50 - 1/100. |
ターゲット情報
-
機能
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
配列類似性
Contains 2 RRM (RNA recognition motif) domains. -
翻訳後修飾
Arg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation. -
細胞内局在
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. - Information by UniProt
-
参照データベース
- Entrez Gene: 3184 Human
- Entrez Gene: 11991 Mouse
- Entrez Gene: 79256 Rat
- GenBank: NP_112738.1 Human
- Omim: 601324 Human
- SwissProt: Q14103 Human
- SwissProt: Q60668 Mouse
- SwissProt: Q9JJ54 Rat
see all -
別名
- ARE binding protein AUFI type A antibody
- AU-rich element RNA-binding protein 1 antibody
- AUF antibody
see all
画像
-
All lanes : Anti-hnRNP D/AUF1 antibody (ab61193) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunizing peptide
Predicted band size: 38 kDa
Observed band size: 38 kDa -
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab61193 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
-
ICC/IF image of ab61193 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61193, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (25)
ab61193 は 25 報の論文で使用されています。
- Oe S et al. Cpeb1 expression is post-transcriptionally regulated by AUF1, CPEB1, and microRNAs. FEBS Open Bio 12:82-94 (2022). PubMed: 34480525
- Puvvula PK & Moon AM Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and Survival. Front Oncol 11:621825 (2021). PubMed: 33859938
- Suzuki T et al. Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver Development. Int J Mol Sci 21:N/A (2020). PubMed: 33297405
- Xu C et al. A novel ZRS variant causes preaxial polydactyly type I by increased sonic hedgehog expression in the developing limb bud. Genet Med 22:189-198 (2020). PubMed: 31395945
- Qian W et al. Angelica Sinensis Polysaccharide Suppresses Epithelial-Mesenchymal Transition and Pulmonary Fibrosis via a DANCR/AUF-1/FOXO3 Regulatory Axis. Aging Dis 11:17-30 (2020). PubMed: 32010478