製品の概要
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製品名Anti-HMGN3 antibody
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製品の詳細Rabbit polyclonal to HMGN3
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由来種Rabbit
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アプリケーション適用あり: WB, IP, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide from a region between residue 60 and 99 of human HMGN3, NP_004233.1.
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ポジティブ･コントロール
- Whole cell lysate from Hela cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab72233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.
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組織特異性Expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries.
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配列類似性Belongs to the HMGN family.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 100069315 Horse
- Entrez Gene: 9324 Human
- Omim: 604502 Human
- SwissProt: Q15651 Human
- Unigene: 77558 Human
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別名
- DKFZp686E20226 antibody
- FLJ42187 antibody
- High mobility group nucleosomal binding domain 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN3 antibody (ab72233)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling HMGN3 with ab72233 at 1/1000 (0.2µg/ml). Detection: DAB. -
Western blot - HMGN3 antibody (ab72233)All lanes : Anti-HMGN3 antibody (ab72233) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 11 kDa
Observed band size: 19 kDa (why is the actual band size different from the predicted?)
Additional bands at: 15 kDa, 16 kDa, 51 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - HMGN3 antibody (ab72233)Immunoprecipitation/ Western Blot of HMGN3.
Lane 1: ab72233 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72233 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
参考文献
This product has been referenced in:
- Yoffe Y et al. Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes Dev 30:1991-2004 (2016). Read more (PubMed: 27664238) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"