製品の概要

  • 製品名Anti-HMGN2 antibody [EPR7091]
    HMGN2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR7091] to HMGN2
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HMGN2.

  • ポジティブ・コントロール
    • HL60, K562, HeLa, SH-SY5Y and Jurkat cell lysates; Human colon tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR7091
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
    • 配列類似性Belongs to the HMGN family.
    • 翻訳後修飾Phosphorylation favors cytoplasmic localization.
    • 細胞内局在Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • High mobility group (nonhistone chromosomal) protein 17 antibody
      • high mobility group nucleosomal binding domain 2 antibody
      • High mobility group nucleosome-binding domain-containing protein 2 antibody
      • High mobility group protein N2 antibody
      • HMG17 antibody
      • HMGN2 antibody
      • HMGN2_HUMAN antibody
      • MGC5629 antibody
      • Non histone chromosomal protein HMG 17 antibody
      • Non-histone chromosomal protein HMG-17 antibody
      • Nonhistone chromosomal protein HMG 17 antibody
      • nonhistone chromosomal protein hmg-17 antibody
      see all

    Anti-HMGN2 antibody [EPR7091] 画像

    • All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : SH-SY5Y cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 9 kDa
    • ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Anti-HMGN2 antibody [EPR7091] (ab124997) 使用論文

    ab124997 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124997.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"