製品の概要

  • 製品名Anti-HMGCS1 antibody
    HMGCS1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to HMGCS1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-520 of Human HMGCS1

  • ポジティブ・コントロール
    • Human liver tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Detects bands of approximately 57 and 65 kDa (predicted molecular weight: 57 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
    • パスウェイMetabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
    • 配列類似性Belongs to the HMG-CoA synthase family.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody
      • 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
      • cytoplasmic antibody
      • EC 2.3.3.10 antibody
      • HMCS1_HUMAN antibody
      • HMG CoA synthase antibody
      • HMG-CoA synthase antibody
      • HMGCS antibody
      • HMGCS1 antibody
      • Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody
      • Hydroxymethylglutaryl-CoA synthase antibody
      • MGC90332 antibody
      see all

    Anti-HMGCS1 antibody 画像

    • Anti-HMGCS1 antibody (ab67242) at 1/500 dilution + human liver tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 57 kDa
    • All lanes : Anti-HMGCS1 antibody (ab67242) at 1/500 dilution

      Lane 1 : HMGCS1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

    Anti-HMGCS1 antibody (ab67242) 使用論文

    This product has been referenced in:
    • Martinez-Outschoorn UE  et al. Ketone bodies and two-compartment tumor metabolism: stromal ketone production fuels mitochondrial biogenesis in epithelial cancer cells. Cell Cycle 11:3956-63 (2012). Read more (PubMed: 23082721) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.
    We have antibodies to HMG CoA Synthase (HMGS) 3 Hydroxy 3 Methyl Glutaryl CoA Synthase only. The catalogue numbers are ab14302; http://www.abcam.com/HMGCS2-antibody-ab14302.html
    ab67242; http://www.abcam.com/HMGCS...

    Read More
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (diffuse large B cell lymphoma cell line)
    Loading amount 1e+006 cells
    Specification diffuse large B cell lymphoma cell line
    Gel Running Conditions Reduced Denaturing (9%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Nov 20 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"