Anti-HMGCL 抗体 (ab97293)
Key features and details
- Rabbit polyclonal to HMGCL
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HMGCL antibody
HMGCL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HMGCL -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 185 of Human HMGCL (NP_000182).
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ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified by antigen affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97293の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Involved in the catabolism of branched amino acids such as leucine. -
組織特異性
Fibroblasts, liver and lymphoblasts. -
パスウェイ
Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1. -
関連疾患
Defects in HMGCL are the cause of 3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD) [MIM:246450]; also known as hydroxymethylglutaricaciduria or HL deficiency. An autosomal recessive disease affecting ketogenesis and L-leucine catabolism. The disease usually appears in the first year of life after a fasting period and its clinical acute symptoms include vomiting, seizures, metabolic acidosis, hypoketotic hypoglycemia and lethargy. These symptoms sometimes progress to coma, with fatal outcome in some cases. -
配列類似性
Belongs to the HMG-CoA lyase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 317658 Cow
- Entrez Gene: 3155 Human
- Entrez Gene: 15356 Mouse
- Entrez Gene: 79238 Rat
- Omim: 246450 Human
- Omim: 613898 Human
- SwissProt: P35914 Human
- SwissProt: P38060 Mouse
see all -
別名
- 3 hydroxy 3 methylglutaryl CoA lyase antibody
- 3 hydroxy 3 methylglutaryl Coenzyme A lyase antibody
- 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase (hydroxymethylglutaricaciduria) antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97293 は 1 報の論文で使用されています。
- Son SM et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor. Nat Commun 11:3148 (2020). PubMed: 32561715