Anti-HMGB4 抗体 (ab65980)
Key features and details
- Rabbit polyclonal to HMGB4
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HMGB4 antibody
HMGB4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HMGB4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human HMGB4 aa 24-41 (N terminal). The human HMGB4 is different to the related mouse and rat sequence by a single amino acid.
Sequence:RNKFKEQQPNTYVGFKEF
Database link: Q8WW32 -
ポジティブ・コントロール
- WB: Rat testicle, brain tissue lysates. Jurkat, HT1080 cell lysates;
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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関連性
High Mobility Group (HMG) proteins, a group of chromosomal proteins common to all eukaryotes, bind DNA in a non-sequence-specific fashion to promote chromatin function and gene regulation, helping with transcription, replication, recombination, and DNA repair. HMGB4 is HMG2-like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, and is found in a variety of eukaryotic chromosomal proteins. HMGB proteins are phosphorylated to various extents. The existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 127540 Human
- Entrez Gene: 685271 Rat
- SwissProt: Q8WW32 Human
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別名
- High mobility group box 4 antibody
- High mobility group protein B4 antibody
- HMG2 like antibody
画像
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All lanes : Anti-HMGB4 antibody (ab65980) at 1/2 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat testicular tissue lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HT1080 whole cell lysate
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Anti-HMGB4 antibody (ab65980) at 1 µg/ml + rat testicle tissue lysis at 30 µg
Predicted band size: 22 kDa
Observed band size: 22 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab65980 は 1 報の論文で使用されています。
- Zhang Z et al. HMGB3 promotes growth and migration in colorectal cancer by regulating WNT/ß-catenin pathway. PLoS One 12:e0179741 (2017). WB ; Human . PubMed: 28678825