製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HMGB2
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Pig
  • 免疫原

    Synthetic peptide:

    KSEAGKKGPGRPTGSK

    , corresponding to amino acids 166-181 of Human HMGB2.

  • ポジティブ・コントロール
    • K-562 nuclear extract.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.2% Gelatin, 0.01% Thimerosal and 0.1% Sodium Azide.
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa.

ターゲット情報

画像

  • Detection of HMG2 by Western blot analysis. HMG2 is detected in nuclear extracts derived from the human K-562 cell line using HMG2 rabbit polyclonal antibody. Lanes 1-3 represent serial dilutions of the antibody (1/500, 1/1000 and 1/2000 respectively).
  • IHC image of ab11973 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11973, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »
  • Syed N  et al. Silencing of high-mobility group box 2 (HMGB2) modulates cisplatin and 5-fluorouracil sensitivity in head and neck squamous cell carcinoma. Proteomics 15:383-93 (2015). Read more (PubMed: 25327479) »

See all 10 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (growth plate)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% tritonx100
Specification
growth plate
Blocking step
bloxall, then serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Apr 27 2017

This HMGB2 ab is directed against HMGB2 and not HMGB1. It does not cross-react.

Thank you for your enquiry. The concentration of the antibody is 0.021 mg/ml. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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