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Our Abpromise guarantee covers the use of ab11354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Antigen retrieval is not necessary from customer feedback.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Block with 5% milk for 45 min. Incubate antibody in PBS/Tween 20/0.25% milk. ab11354 was tested in IF using HeLa cells but gave widespread cytoplasmic as well as nuclear staining, and thus may not be suitable for this application.|