製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HMGA2
  • アプリケーション
    適用あり: IHC-P, IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474).

  • ポジティブ・コントロール
    • MOLT4 whole cell lysate, MCF7 cells, HepG2, HCT116, HeLa

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • 関連疾患
    Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • 配列類似性
    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • 発生段階
    Expressed predominantly during embryogenesis.
  • 翻訳後修飾
    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Anti-HMGA2 antibody 画像

  • Lane 1: Input

    Lane 2: Immunoprecipitation using control IgG

    Lane 3: Immunoprecipitation using 5 μg ab97276 

  • Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 12 kDa
    15% SDS PAGE
  • ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

    Lane 1 : HepG2
    Lane 2 : HCT116

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 12 kDa
  • Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution

    Predicted band size : 12 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an anonymous abreview.

    See Abreview

Anti-HMGA2 antibody (ab97276) 使用論文

This product has been referenced in:
  • Wang H  et al. MicroRNA-495 Inhibits Gastric Cancer Cell Migration and Invasion Possibly via Targeting High Mobility Group AT-Hook 2 (HMGA2). Med Sci Monit 23:640-648 (2017). WB ; Human . Read more (PubMed: 28159956) »
  • Galicia-Vázquez G  et al. eIF4AII is dispensable for miRNA-mediated gene silencing. RNA 21:1826-33 (2015). Read more (PubMed: 26286746) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (Primary Fetal Fibroblast)
Gel Running Conditions
Reduced Denaturing (Mini-PROTEAN® TGX™ 4-20%)
Loading amount
50 µg
Specification
Primary Fetal Fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Nov 21 2016

Application
ChIP
Detection step
Semiquantitative PCR
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Negative control
DW No-antibody
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
input (whole lysate)
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Abcam user community

Verified customer

投稿 Sep 19 2014

The predicted size of HMGA2 is at 12 kDa, but it is also reported to migrate at higher size around 17-19 kDa probably due to PTM . (see publication: http://www.ncbi.nlm.nih.gov/pubmed/15026339)

The Uniprot database also shows HMGA2 has severa...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLA)
Loading amount
25 µg
Specification
HeLA
Gel Running Conditions
Reduced Denaturing (17.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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