製品の概要

  • 製品名Anti-HMGA2 antibody
    HMGA2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to HMGA2
  • アプリケーション適用あり: ICC, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: MSARGEGAGQ PSTSAQGQPA APAPQKRGRG RPRKQQQEPT GEPSPKRPRG RPKGSKNKSP SKAAQKKAEA TGEKRPRGRP RKWPQQVVQK KP, corresponding to amino acids 1-93 of Human HMGA2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 18802962
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • 関連疾患Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • 配列類似性Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • 発生段階Expressed predominantly during embryogenesis.
  • 翻訳後修飾Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Anti-HMGA2 antibody 画像

  • Overlay histogram showing HepG2 cells stained with ab56390 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56390, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab56390 staining HMGA2 in HeLa cells.

Anti-HMGA2 antibody (ab56390) 使用論文

This product has been referenced in:
  • Jeng YM  et al. RNA-binding protein insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor invasion and predicts early recurrence and poor prognosis in hepatocellular carcinoma. Hepatology 48:1118-27 (2008). IHC-P ; Human . Read more (PubMed: 18802962) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I can recommend to review the online datasheets for further information. Prices are provided at the top of the online datasheets. Read More

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U20S Cell Line)
Loading amount 200 µg
Specification U20S Cell Line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"