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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HMGA1a/ HMGA1b.
(Peptide available as ab20073.)
Our Abpromise guarantee covers the use of ab4078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa).|
|ChIP||Use at an assay dependent concentration.
See Abreview submitted on 15 December 2009.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/250 - 1/500.
Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below.
Immunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.
A: Hoechst stain
C: TexasRed HMG-I/HMG-Y (ab4078)
Western blot using ab4078.
Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-, hHMGA1b-, hHMGA2- or NLS- (nuclear localisation signal) GFP fusion proteins.
ab4078 was diluted 1/1000 in 5% milk/TBS and incubated for 1 hr at room temperature. Secondary HRP anti-rabbit antibody was diluted 1/10000 in 5% milk/TBS and incubated for 1 hr at room temperature.
Lane 1: hHMGA1a-GFP (ab4078)
Lane 2: hHMGA1b-GFP (ab4078)
Lane 3: hHMGA2-GFP (ab4078)
Lane 4: NLS-GFP (ab4078)
Lane 5: hHMGA1a-GFP (anti-GFP)
Lane 6: hHMGA1b-GFP (anti-GFP)
Lane 7: hHMGA2-GFP (anti-GFP)
Lane 8: NLS-GFP (anti-GFP)
The detected size is that expected for the fusion of an 11kD and 26kD protein (GFP).