製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HMG4
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Binds preferentially single-stranded DNA and unwinds double stranded DNA.
  • 組織特異性
    Expressed predominantly in placenta.
  • 配列類似性
    Belongs to the HMGB family.
    Contains 2 HMG box DNA-binding domains.
  • 細胞内局在
    Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • chromosomal protein, Nonhistone, HMG4 antibody
    • High mobility group (nonhistone chromosomal) protein 4 antibody
    • High mobility group box 3 antibody
    • High mobility group protein 2a antibody
    • High mobility group protein 4 antibody
    • High mobility group protein B3 antibody
    • High mobility group protein HMG4 antibody
    • HMG 4 antibody
    • HMG-2a antibody
    • HMG-4 antibody
    • HMG2A antibody
    • HMGB 3 antibody
    • Hmgb3 antibody
    • HMGB3_HUMAN antibody
    • MGC90319 antibody
    • Non histone chromosomal protein antibody
    • Nonhistone chromosomal protein HMG4 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-HMG4 antibody (ab72544) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

参考文献

ab72544 has not yet been referenced specifically in any publications.

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