ab34760 recognises human HMGN pS20/pS24 proteins and the antibody is specific for the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA.
The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/25000.
1/500 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
Belongs to the HMGN family.
Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
ab34760 at 20 µg/ml staining of epidermal keratinocytes at 40X. This image shows moderate nuclear and faint cytoplasmic positive staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.