製品の概要

  • 製品名Anti-HMG14 antibody
    HMG14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HMG14
  • アプリケーション適用あり: IP, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 50-92 of Human HMG14 (NP_004956.5)

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab114127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
    • 配列類似性Belongs to the HMGN family.
    • 翻訳後修飾Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
    • 細胞内局在Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ27265 antibody
      • FLJ31471 antibody
      • High mobility group (nonhistone chromosomal) protein 14 antibody
      • High mobility group nucleosome binding 1 antibody
      • High mobility group nucleosome binding domain 1 antibody
      • High mobility group nucleosome binding domain containing protein 1 antibody
      • High mobility group nucleosome-binding domain-containing protein 1 antibody
      • High mobility group protein 14 antibody
      • HMG 14 antibody
      • HMG14 antibody
      • HMGN 1 antibody
      • HMGN1 antibody
      • HMGN1_HUMAN antibody
      • MGC104230 antibody
      • MGC117425 antibody
      • Non-histone chromosomal protein HMG-14 antibody
      • Nonhistone chromosomal protein HMG 14 antibody
      • Nonhistone chromosomal protein HMG14 antibody
      see all

    Anti-HMG14 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HMG14 with ab114127 at 1/1000 (1µg/ml). Detection: DAB.
    • ab114127 at 10ug/mg of lysate detecting HMG14 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
      Detection: chemiluminescence. Exposure time: 30 seconds.

    Anti-HMG14 antibody (ab114127) 使用論文

    ab114127 has not yet been referenced specifically in any publications.

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