The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/4000. Detects a band of approximately 6 kDa (predicted molecular weight: 6 kDa).
hliA and hliB proteins are LHC similar stress induced proteins from cyanobacteria. This group of proteins is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.
high light inducible protein antibody
Western blot - hliA antibody (ab65480)
All lanes : Anti-hliA antibody (ab65480) at 1/2000 dilution
Lane 1 : Synechocystis sp. PCC 6803 membrane proteins extracted from cells acclimated to low light regimes Lane 2 : Synechocystis sp. PCC 6803 membrane proteins extracted from cells acclimated to high light regimes
Lysates/proteins at 3 µg per lane.
Secondary All lanes : anti-rabbit at 1/30000 dilution
Developed using the ECL technique.
Predicted band size: 6 kDa Observed band size: 6 kDa Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.