製品の概要

  • 製品名Anti-HLA G antibody [MEM-G/9] (FITC)
    HLA G 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-G/9] to HLA G (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性Reacts with native form of human HLA-G on the cell surface. Specific reaction was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Non Human Primates
  • 免疫原

    Recombinant human HLA G refolded with beta 2 microglobulin and peptide.

  • ポジティブ・コントロール
    • JEG-3 human choriocarcinoma cell line
  • 特記事項Avoid prolonged exposure to light.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)The antibody was purified from ascites by precipitation methods (purity > 95 % by PAGE) and conjugated with fluorescein isothiocyanate (FITC). Excess of FITC was removed by dialysis against PBS containing 15mM sodium azide.
  • ポリ/モノモノクローナル
  • クローン名MEM-G/9
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 20713594
Flow Cyt 1/500. Do not fix cells (see Menier et al).



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 組織特異性Expressed in trophoblasts.
  • 配列類似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Anti-HLA G antibody [MEM-G/9] (FITC) (ab7904) 使用論文

This product has been referenced in:
  • La Manna G  et al. Neutrophil gelatinase-associated lipocalin increases HLA-G(+)/FoxP3(+) T-regulatory cell population in an in vitro model of PBMC. PLoS One 9:e89497 (2014). Flow Cyt ; Human . Read more (PubMed: 24586826) »
  • Zhao L  et al. Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens 80:231-8 (2012). Read more (PubMed: 22738368) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us.

As discussed yesterday please buy ab18435 as isotype control.

The clone has been mentioned in many publications, most of the time the cells have been permeabilized so I would suggest permeabilizing the ...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

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Thank you foryoru enquiry.

Regret to inform you that we have no experiences with intracellular staining of ab7904: HLA-G. We have not tested this and we do not have a proved protocol for this. In general, when we need to perform intracellular...

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I would suggest that you should carry out a preliminary experiment to test different dilutions of the primary antibody (i.e. 0.1-10 microgram/ml) and then adjust the dilution accordingly to the results obtained.

As this antibody is conjugated to FITC we do not recommend that you freeze the antibody. Store at 2-8 deg C in the dark - it will be stable for much longer than 1 month.

This antibody is currently in stock, so we can dispatch it within 24 hours of receiving your order (unless we receive it on a Friday, in which case we will dispatch on Monday to avoid weekend delivery). The antibody is stable for at least 1 month at 4 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"