製品の概要

  • 製品名Anti-HLA G antibody [G233]
    HLA G 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [G233] to HLA G
  • 特異性ab52454 recognises several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). It does not cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).
  • アプリケーション適用あり: ICC/IF, IP, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Murine L cells transfected with both human beta2-microglobulin and HLA-G.

  • ポジティブ・コントロール
    • Jeg3 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名G233
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 - 10 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 組織特異性Expressed in trophoblasts.
  • 配列類似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Anti-HLA G antibody [G233] 画像

  • ICC/IF image of ab52454 stained Jeg3 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52454, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HLA G antibody [G233] (ab52454) 使用論文

This product has been referenced in:
  • Cho S  et al. The C-terminal amino acid of the MHC-I heavy chain is critical for binding to Derlin-1 in human cytomegalovirus US11-induced MHC-I degradation. PLoS One 8:e72356 (2013). Human . Read more (PubMed: 23951315) »
  • Gonzalez A  et al. Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates. Clin Chem 57:1013-22 (2011). Read more (PubMed: 21527645) »

See all 6 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab52454.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"