製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    >95% by SDS-PAGE
  • 特記事項（精製）
    Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • ポリ/モノ
    モノクローナル
  • クローン名
    4H84
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 組織特異性
    Expressed in trophoblasts.
  • 配列類似性
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [4H84] (ab52455)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [4H84] (ab52455)This image is courtesy of an Abreview submitted by Mr Rudolf Jung
      ab52455 staining HLA G in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized Wash Buffer with Tween; antigen retrieval was by heat mediation in a TRIS-EDTA Buffer (pH 9.0). Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Goat anti-Mouse HRP polyclonal was used as the secondary antibody.

      See Abreview

    参考文献

    This product has been referenced in:
    • Horii M  et al. Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease. Proc Natl Acad Sci U S A 113:E3882-91 (2016). ICC/IF . Read more (PubMed: 27325764) »
    • Jasinski-Bergner S  et al. Clinical relevance of miR-mediated HLA-G regulation and the associated immune cell infiltration in renal cell carcinoma. Oncoimmunology 4:e1008805 (2015). Read more (PubMed: 26155421) »

    See all 7 Publications for this product

    ab52455 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    8 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HLA G antibody [4H84]

     Good
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Human Tissue sections (Human placental explants)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Human placental explants
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Angela Crespo

    Verified customer

    投稿 Sep 15 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA G antibody [4H84]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (First trimester decidua)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate
    Permeabilization
    No
    Specification
    First trimester decidua
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Ruhul Choudhury

    Verified customer

    投稿 Jan 24 2017

    Western blot abreview for Anti-HLA G antibody [4H84]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (NPC)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    NPC
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jan 09 2017

    antibody for IHC-P on human and rat

    Merci de votre appel de toutà l'heure.

    Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA G antibody [4H84]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer (pH 9.0)
    Permeabilization
    Yes - DAKO Wash-Buffer with Tween
    Read More
    abreview image
    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 May 01 2012

    This antibody is giving us unecpected bands at 40 and 60 kDa. I would like to try ab7759 with my samples

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

    Read More

    Flow Cytometry abreview for Anti-HLA G antibody [4H84]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (JEG-3)
    Specification
    JEG-3
    Fixation
    None
    Permeabilization
    No
    Gating Strategy
    iostype control
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 May 17 2010

    Western blot abreview for Anti-HLA G antibody [4H84]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (JEG-3)
    Loading amount
    30 µg
    Specification
    JEG-3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 May 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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