Customer reviews and FAQs

製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項（精製）Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名4H84
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • 組織特異性Expressed in trophoblasts.
  • 配列類似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    Anti-HLA G antibody [4H84] 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [4H84] (ab52455)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [4H84] (ab52455)This image is courtesy of an Abreview submitted by Mr Rudolf Jung
      ab52455 staining HLA G in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized Wash Buffer with Tween; antigen retrieval was by heat mediation in a TRIS-EDTA Buffer (pH 9.0). Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Goat anti-Mouse HRP polyclonal was used as the secondary antibody.

      See Abreview

    Anti-HLA G antibody [4H84] (ab52455) 使用論文

    This product has been referenced in:
    • Sugimoto J  et al. A novel human endogenous retroviral protein inhibits cell-cell fusion. Sci Rep 3:1462 (2013). IHC-P ; Human . Read more (PubMed: 23492904) »
    • Li Y  et al. BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state. Development 140:3965-76 (2013). Human . Read more (PubMed: 24004950) »

    See all 4 Publications for this product

    ab52455 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab52455 の abreview を投稿する ab52455 に関する問い合わせをする

    7 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA G antibody [4H84]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (First trimester decidua)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
    Permeabilization No
    Specification First trimester decidua
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Formaldehyde
    Read More
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    Username

    Ruhul Choudhury

    Verified customer

    投稿 Jan 24 2017

    Western blot abreview for Anti-HLA G antibody [4H84]

     Poor
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (NPC)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification NPC
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Jan 09 2017

    antibody for IHC-P on human and rat

    Merci de votre appel de toutà l'heure.

    Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA G antibody [4H84]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (spleen)
    Specification spleen
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer (pH 9.0)
    Permeabilization Yes - DAKO Wash-Buffer with Tween
    Read More
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    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 May 01 2012

    This antibody is giving us unecpected bands at 40 and 60 kDa. I would like to try ab7759 with my samples

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

    Read More

    Flow Cytometry abreview for Anti-HLA G antibody [4H84]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (JEG-3)
    Specification JEG-3
    Fixation None
    Permeabilization No
    Gating Strategy iostype control
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 May 17 2010

    Western blot abreview for Anti-HLA G antibody [4H84]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (JEG-3)
    Loading amount 30 µg
    Specification JEG-3
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 May 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"