製品の概要

  • 製品名
    Anti-HLA E antibody [MEM-E/07]
    HLA E 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-E/07] to HLA E
  • 特異性
    The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly).
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    Purified from hybridoma culture supernatant by protein A-affinity chromatography.
  • ポリ/モノ
    モノクローナル
  • クローン名
    MEM-E/07
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 20713594
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • HLA class I histocompatibility antigen E alpha chain antibody
    • EA1.2 antibody
    • EA2.1 antibody
    • HLA 6.2 antibody
    • HLA class I histocompatibility antigen alpha chain E antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA6.2 antibody
    • HLAE antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I E antibody
    • MHC antibody
    • MHC class I antigen E antibody
    • MHC HLA E alpha 1 antibody
    • MHC HLA E alpha 2.1 antibody
    • QA1 antibody
    see all

画像

  • ab11820 staining HLA E in Human endothelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/100 in 5% BSA in PBS with 0.1% Triton X-100) for 1 hour at 4°C. An undiluted FITC-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Cells were treated with 500 Units of human IFN-gamma on 1 million ECV304 cells after 36 hours cells were harvested (PVG 0.1% trypsin), fixed with 2% paraformaldehyde and surface staining of HLA E was performed using ab11820 at a 1/100 dilution. The secondary used was a goat anti-mouse-FITC, at a 1/100 dilution.
    Red line indicates the untreated expression level and black indicate the induced surface expression of HLA E. Gating strategy - total population.

    See Abreview

  • Image from Park B et al, J Exp Med. 2010 Aug 30;207(9):2033-41. Epub 2010 Aug 16, Fig S1. DOI 10.1084/jem.20091793

    HLA-G– or HLA-E–expressing HeLa cells were transfected with HCMV endoplasmic reticulum resident transmembrane glycoprotein US10. The lysates were immunoblotted with antibodies against HLA-G, HLA-E (ab11820), or beta-actin as a loading control. The figure shows that expression of HLA-G is slightly reduced by US10.

参考文献

This product has been referenced in:
  • Shwetank  et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). Read more (PubMed: 25086398) »
  • Shwetank  et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . Read more (PubMed: 24236107) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Human Cell (Human endothelial cell line)
Specification
Human endothelial cell line
Permeabilization
Yes - 0.1% triton-x 100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial)
Loading amount
100 µg
Specification
endothelial
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Human Endothelial cell line (ECV304))
Specification
Human Endothelial cell line (ECV304)
Preparation
Cell harvesting/tissue preparation method: Trypsinisation
Sample buffer: PVG containing 0.1% trypsin
Fixation
2% Paraformaldehyde in PBS
Permeabilization
No
Gating Strategy
total population
Username

Mr. Shwetank Verma

Verified customer

投稿 Mar 16 2011

I am pleased to be able to provide you with two references: Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003). Palmisano GL et al. HLA-E surface expression is independent of t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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