製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to HLA E
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human HLA E protein (AAH02578.1).

  • ポジティブ・コントロール
    • 293 cells, Human spleen tissue lysate and transfected 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • HLA class I histocompatibility antigen E alpha chain antibody
    • EA1.2 antibody
    • EA2.1 antibody
    • HLA 6.2 antibody
    • HLA class I histocompatibility antigen alpha chain E antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA6.2 antibody
    • HLAE antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I E antibody
    • MHC antibody
    • MHC class I antigen E antibody
    • MHC HLA E alpha 1 antibody
    • MHC HLA E alpha 2.1 antibody
    • QA1 antibody
    see all

画像

  • FACS analysis of negative control 293 cells (Black) and HLA E expressing 293 cells (Green) using ab88090.
  • Anti-HLA E antibody (ab88090) at 5 µg/ml + Human spleen tissue lysate at 50 µg

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • All lanes : Anti-HLA E antibody (ab88090) at 5 µg/ml

    Lane 1 : 293T cell line (HLA E transfected lysate)
    Lane 2 : 293T cell line (Non-transfected lysate)

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa

参考文献

ab88090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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