製品の概要

  • 製品名Anti-HLA DRB5 antibody [MRC OX-17]
    HLA DRB5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MRC OX-17] to HLA DRB5
  • 特異性Does not bind to thymocytes or erythrocytes.
    Does not cross-react with rat Ia-A or mouse Ia-E antigen, but rabbit antibody raised against the antibody affinity column-purified MRC OX-17 antigen cross-reacted on tissues of mice expressing Ia-E mouse antigen but not on those mouse strains that were Ia-E antigen negative.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Rat spleen membrane glycoproteins depleted of Ia-A antigens.

  • ポジティブ・コントロール
    • Thymus (12% positive cells); Spleen (34% positive cells); Lymph Node (25% positive cells). Wistar, Buffalo, Brown Norway, ACI, and Fischer 344 stains.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名MRC OX-17
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/250 - 1/500. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 配列類似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 翻訳後修飾Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to down-regulation of MHC class II.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DR 9 antibody
    • DR beta 5 antibody
    • DR beta 5 chain antibody
    • DR beta-5 antibody
    • DR beta5 antibody
    • DR2 beta 2 antibody
    • DR2-beta-2 antibody
    • DR9 antibody
    • DRB5_HUMAN antibody
    • Dw2 antibody
    • FLJ76359 antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR beta 5 chain antibody
    • HLA class II histocompatibility antigen DRB1-9 beta chain antibody
    • HLA class II histocompatibility antigen, DR-5 beta chain antibody
    • HLA DRB antibody
    • HLA-DRB5 antibody
    • Human leucocyte antigen DRB5 antibody
    • Leukocyte antigen class II antibody
    • Major histocompatibility complex, class II, DR beta 51 antibody
    • MHC class II antigen DRB1*9 antibody
    • MHC class II antigen DRB5 antibody
    • MHC class II HLA beta chain antibody
    • MHC HLA-DR-beta cell surface glycoprotein antibody
    • MHC HLA-DR-beta chain antibody
    see all

Anti-HLA DRB5 antibody [MRC OX-17] 画像

  • Rat Strain: Lewis Rat
    Cell Concentration: 1x106 cells
    Antibody Concentration: 1:400
    Isotypic Control: Mouse IgG , k
    Cell Source: Splenocytes
    Percentage of Cells Stained Above Control: 34% .

Anti-HLA DRB5 antibody [MRC OX-17] (ab119795) 使用論文

This product has been referenced in:
  • Fukumoto T  et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). Read more (PubMed: 6178598) »

See 1 Publication for this product

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