Anti-HLA DR + DP + DQ 抗体 [WR18] (Phycoerythrin) (ab23901)

製品の概要

  • 製品名Anti-HLA DR + DP + DQ antibody [WR18] (Phycoerythrin)
    HLA DR + DP + DQ 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [WR18] to HLA DR + DP + DQ (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性ab23901 recognises the common b unit of HLA-Class 2 molecules. Stains B cells, monocytes and activated T cells.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    B-CLL cells (Human).

  • 特記事項

    Purified IgG conjugated to R. Phycoerythrin (RPE).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名WR18
  • ミエローマNS1
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. (or 100ul whole blood).



ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 配列類似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD antibody
    • CELIAC 1 antibody
    • CELIAC1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DQ A1 antibody
    • DRB1 antibody
    • DRB4 antibody
    • FLJ27088 antibody
    • FLJ27328 antibody
    • GSE antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR 1 beta antibody
    • HLA class II histocompatibility antigen DR alpha antibody
    • HLA class II histocompatibility antigen DR alpha chain antibody
    • HLA DP histocompatibility type beta 1 subunit antibody
    • HLA DP1B antibody
    • HLA DPB1 antibody
    • HLA DQA antibody
    • HLA DQA1 antibody
    • HLA DQB antibody
    • HLA DQB1 antibody
    • HLA DR1B antibody
    • HLA DR3B antibody
    • HLA DR4B antibody
    • HLA DRA antibody
    • HLA DRA1 antibody
    • HLA DRB1 antibody
    • HLA DRB3 antibody
    • HLA DRB4 antibody
    • HLA DRB5 antibody
    • HLA-DPB1 antibody
    • IDDM 1 antibody
    • IDDM1 antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • Major histocompatibility complex class II DQ alpha 1 antibody
    • Major histocompatibility complex class II DQ beta 1 antibody
    • Major histocompatibility complex class II DR alpha antibody
    • Major histocompatibility complex class II DR beta 1 antibody
    • Major histocompatibility complex class II DR beta 3 antibody
    • Major histocompatibility complex class II DR beta 4 antibody
    • Major histocompatibility complex class II DR beta 5 antibody
    • MGC117330 antibody
    • MHC class II antigen DPB1 antibody
    • MHC class II antigen DRA antibody
    • MHC class II HLA DQ alpha 1 antibody
    • MHC class II HLA DR beta 1 antibody
    • MHC class II HLA DR beta 3 antibody
    • MHC DPB1 antibody
    • MHC DQ beta antibody
    • MHC HLA DPB1 antibody
    • MHC HLA DQ alpha antibody
    see all

Anti-HLA DR + DP + DQ antibody [WR18] (Phycoerythrin) (ab23901) 使用論文

This product has been referenced in:
  • Brignone C  et al. A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells. J Immunol 179:4202-11 (2007). Flow Cyt ; Human . Read more (PubMed: 17785860) »

See 1 Publication for this product

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Thank you for your enquiry. Unfortunately, there's no concentration available for this conjugate antibody. The antibody's originator recommends using the antibody neat for application in FACS. Please contact us again if you have any additional quest...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"