Anti-HLA-DR 抗体 [EPR3691(2)] (ab124809)

製品の概要

• 製品名
  Anti-HLA-DR antibody [EPR3691(2)]
  HLA-DR 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EPR3691(2)] to HLA-DR
• アプリケーション
  適用あり: WBmore details
  適用なし: Flow Cyt,ICC,IHC-P or IP
• 種交差性
  交差種: Human
  非交差種: Mouse, Rat
• 免疫原

  corresponding to Human HLA-DR aa 100-200.

• ポジティブ･コントロール
  • Raji, Ramos, HuT-78 and Human spleen lysates
• 特記事項

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• バッファー
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 精製度
  Tissue culture supernatant
• ポリ/モノ
  モノクローナル
• クローン名
  EPR3691(2)
• アイソタイプ
  IgG
• 研究分野
  • Immunology
  • Adaptive Immunity
  • MHC
  • Class II

関連製品

• Positive Controls
  • Raji whole cell lysate (ab30124)
  • Raji membrane extract lysate (ab30126)
  • Ramos whole cell lysate (ab3955)

アプリケーション

ターゲット情報

• 機能
  Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
• 配列類似性
  Belongs to the MHC class II family.
  Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
• 翻訳後修飾
  Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
• 細胞内局在
  Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

• Target information above from: UniProt accession P01903 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 3122 Human
  • Omim: 142860 Human
  • SwissProt: P01903 Human
  • Unigene: 520048 Human
• 別名
  • DR alpha chain antibody
  • DR alpha chain precursor antibody
  • DRA_HUMAN antibody

  • DRB1 antibody
  • DRB4 antibody
  • Histocompatibility antigen HLA DR alpha antibody
  • HLA class II histocompatibility antigen antibody
  • HLA class II histocompatibility antigen DR alpha chain antibody
  • HLA DR1B antibody
  • HLA DR3B antibody
  • HLA DRA antibody
  • HLA DRA1 antibody
  • HLA DRB1 antibody
  • HLA DRB3 antibody
  • HLA DRB4 antibody
  • HLA DRB5 antibody
  • HLA-DRA antibody
  • HLADR4B antibody
  • HLADRA1 antibody
  • HLADRB antibody
  • Major histocompatibility complex class II DR alpha antibody
  • Major histocompatibility complex class II DR beta 1 antibody
  • Major histocompatibility complex class II DR beta 3 antibody
  • Major histocompatibility complex class II DR beta 4 antibody
  • Major histocompatibility complex class II DR beta 5 antibody
  • MGC117330 antibody
  • MHC cell surface glycoprotein antibody
  • MHC class II antigen DRA antibody
  • MHC II antibody
  • MLRW antibody

  see all

プロトコール

• Western blot protocols