The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 20µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens: class I (HLA A, HLA B and HLA C) and class II (HLA D).