製品の概要

  • 製品名Anti-HLA DP antibody
    HLA DP 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to HLA DP
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-260 of human HLA DP (AAH09956.1).

  • ポジティブ・コントロール
    • Human spleen, transfected 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.

ターゲット情報

  • 関連性HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
  • 細胞内局在Type I membrane protein; Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
  • 参照データベース
  • 別名
    • DP(W3) antibody
    • DP(W4) antibody
    • HLA class II histocompatibility antigen DP alpha 1 chain antibody
    • HLA class II histocompatibility antigen DP alpha chain antibody
    • HLA DP1A antibody
    • HLA DPA 1 antibody
    • HLA SB alpha chain antibody
    • HLADP antibody
    • HLADPA1 antibody
    • HLASB antibody
    • HLASB histocompatibility type antibody
    • Human MHC class II HLA SB alpha gene antibody
    • Major histocompatibility complex class II DP alpha 1 antibody
    • MHC class II antigen antibody
    • MHC class II DP3 alpha antibody
    • MHC class II DPA1 antibody
    • MHC class II HLA DPA1 antigen antibody
    • PLT1 antibody
    • Primed lymphocyte test 1 antibody
    see all

Anti-HLA DP antibody 画像

  • Anti-HLA DP antibody (ab88089) at 1 µg/ml + human spleen lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HLA DP antibody (ab88089) at 1 µg/ml

    Lane 1 : transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa

Anti-HLA DP antibody (ab88089) 使用論文

ab88089 has not yet been referenced specifically in any publications.

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Here is a link to the fractionation protocol as discussed: http://www.abcam.com/index.html?pageconfig=resource&rid=11473 I really think that enriching for the membrane fraction may help. I will also discuss with one of my colleagues.

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