特異性Ab34054 reacts to HLA Class 2 Antigen-DR7. Additional cross reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報Flow Cyt: Use at an assay dependent dilution.
This product may also be used in Cell Typing, Tissue Staining, and Chimerism Studies.
For cytotoxicity add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution.
Expected results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.